AI cell analysis offers streamlined solutions for your research projects. Simplify the complexity of classifying live versus dead cells using artificial intelligence (AI)-driven image analysis – with no need for labels. Incucyte® AI Cell Health Analysis Software provides a powerful workflow that supports your entire research team.
The Incucyte® AI Cell Health Analysis Software Module is available to purchase for all S-series instruments and requires a GPU co-processor (BA-04870) installed as a drop-in hardware upgrade to the controller and 2022B software version. Optional further classification of cell populations based on label-free, or fluorescence parameters (fluorescence intensity within cell boundary) is available in this software module.
The Incucyte® Advanced Label-free Classification Analysis Software Module (Cat. No. BA-04867) is an add on module to AI cell Health Analysis module and enables label-free identification of cells with morphologies of interest.
Software Overview
Artificial Intelligence (AI) in Live-Cell Analysis – Overview
Incucyte® analysis now makes use of AI technologies to process label-free images to identify and measure cells utilizing machine learning techniques and neural networks that produce:
- Accurate and robust label-free analysis to determine live versus dead cells – no fluorescent dyes needed
- Objective, repeatable results based on training sets containing millions of cells
- Ever-expanding library of cell types and experiment scenarios
- Simple workflow for all users – less input required
Incucyte® AI Cell Health Analysis Workflow

Incucyte® AI Cell Health Analysis, a neural network (pre-trained with validated datasets) informs a classification algorithm for accurate processing and quantification of live or dead cells. This AI-based classifier is applied to all wells and timepoints, providing robust, segmented data and visualization of live or dead masks.
Incucyte® AI Cell Analysis Software Features
Achieve unbiased, high-quality viability data for a vast array of cell morphologies.
- Utilize integrated AI analysis and smart default settings for classifying live/dead cells and morphological differences
- Classify cell populations based on label-free or fluorescence parameters (fluorescence intensity within cell boundary)
- Track adherent or non-adherent cell populations kinetically and easily visualize changes in viability
- Achieve consistent results with objective analysis using microplate formats, unlocking significant throughput
- Generate reproducible, robust data sets and export data from thousands of analyzed images with intuitive software and less user input
Incucyte® AI Applications

Incucyte® Proliferation Assay (AI Confluence Analysis)
The new Incucyte® AI Confluence algorithm is a robust, AI-driven analysis designed to adapt to a wide range of cell types and morphologies while requiring less user input.

Incucyte® Label-Free Viability Assay
The new Incucyte® AI Cell Health Analysis allows users to increase throughput of cell health analyses without need for fluorescent labels, generating accurate and objective data with reduced time requirements and cost.
