Octet® R4 system for label-free characterization of biomolecules

Octet® R4 has four parallel, independent channels for increased flexibility and faster results. It can be used for a wide range of analyzes including: kinetic analysis, titer of IgG and other proteins, reagent qualification, immunoassay development, bioprocess development, quality analysis, rough antibody screening, docking/epitope mapping, ligand binding assays and small molecule analysis, mechanism elucidation cellular signaling and monitoring of infectious diseases. Analysis can be performed using one to all four channels, allowing flexibility in sample flow.

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Fast results

The Octet^® R4 system has four parallel, independent channels for increased flexibility and faster results.

High sensitivity, moderate throughput, low cost

The Octet^® R4 system is designed for customers looking for moderate throughput sample analysis, without any compromise on assay sensitivity.

Reduced assay time and maintenance cost

The Octet^® platform provides faster time to results and process economy when compared to other technologies due to its fluidics-free Dip and Read format. Additionally, crude sample compatibility saves valuable time by eliminating the need for sample pre-treatment, and non-destructive testing conserves precious samples for other assays.

Sample plate cooling for temperature-sensitive proteins

The sample plate temperature can be controlled from 15–40°C, which enables reliable kinetic determinations at different temperatures for temperature-sensitive proteins. Additional advantages of sample cooling include the ability to rapidly determine binding rate constants at multiple temperatures to extrapolate thermodynamic measurements. The Octet^® R4 system’s four channels can be used independently to measure samples for screening purposes or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.

Kinetic and Affinity Characterization

The Octet^® R4 system monitors binding events in real time to calculate on rates (ka), off rates (kd), and affinity constants (KD) by bringing the detection surface directly to the sample, eliminating the need for microfluidics.

Protein Quantitation in Bioprocessing

Accurately determining the concentration of antibodies and other proteins is critical to the selection of cell lines for development and the optimization of antibody production. The Octet^® R4 system directly measures the presence of specific proteins and other molecules in solution with minimal interference from complex matrices with reproducible concentrations determined in as little as 2 minutes per sample using a simple, one-step assay. Rapid turnaround time of results improves process efficiencies and productivity that cannot be achieved with othermethods such as HPLC and ELISA.